VIA Freeze

GMP-ready units that do not need liquid nitrogen to maintain cell viability and function

Description

Description

Achieve consistent sample quality with three models to suit your processing requirements and scale

The VIA Freeze range of controlled-rate freezers are liquid nitrogen-free. They combine customizable freezing profiles, electronic control systems, and a conduction cooling method to optimize GMP-compliant cryopreservation.

  • Controlled cooling. Maintain cell function and viability with therapy-specific freeze profiles and precise temperature control.
  • Consistent quality. Remove heat evenly from all samples thanks to conduction cooling. Standardize by reliably delivering profiles between networked units.
  • Clean room compatibility. Streamline manufacturing processes. Eliminate risks and infrastructure costs associated with liquid nitrogen.
  • Supports cGMP-compliant cryopreservation. Ready to integrate with cGMP process validation procedures. Controls designed to meet 21 CFR Part 11 regulations.
  • Versatility. Increase productivity without changing processes between VIA Freeze models. Options for 24–192 cryogenic vials, cryobags up to 250 mL, and straws.
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Specifications

Specifications

Volume capacity

Research:48 × 2.0 mL standard cryovials
Duo:96 × 2.0 mL standard cryovials or 125 mL baxter bag(with optional flat sample plate)
Quad:192 × 2.0 mL standard cryovials or 250 mL baxter bag(with optional flat sample plate)

Cleaning

Seamless sample area, no dirt traps, may be cleaned using standard wipes (Shield Medicare – Klerwipe™-CR)

Power

Research:150W
Duo:350W
Quad:600W

Sample plates

Research:1
Duo:2
Quad:4

Dimensions(cm)

Research:29.3(W) x 35.9(D) x 44.9(H)
Duo:44.6(W) x 38.3(D) x 61(H)
Quad:63.8(W) x 38.3(D) x 61(H)

Interface

Secure ethernet interface for remote control in automated applications

Working Temperature

5°C ~ 30°C

Power supply

Research:100-240 V, 50-60 Hz, 2A max
Duo:100-240 V, 50-60 Hz, 3A max
Quad:100-240 V, 50-60 Hz, 5A max

Software

Onboard secure data logging and fully featured protocol editor

Net Weight

Research:14 kg (31 lb)
Duo:40 kg (88 lb)
Quad:64 kg (141 lb)

Minimum temperature

Research:-90°C
Duo:-100°C
Quad:-100°C

Plates and adapters for VIA Freeze

Plates and adapters for VIA Freeze

為達到最佳降溫效果,使用VIA Freeze時請選用合適的樣品盤。目前提供的樣品盤規格如下(若需要其他規格請來電洽詢):

Vial

Small vial SBS plate for VIA Freeze freezers

適用容器:Thermo Fisher™ 1 mL vials 

總乘載量:96 × 1 mL vial 

型號:ASY_30072

Standard size vial SBS plate for VIA Freeze freezers

適用容器:Corning™ 2 mL vial (internally threaded), FluidX 2 mL vial 

總乘載量:48 × 2 mL standard size vial 

型號:ASY_30020

2 mL Aseptic Technologies vial SBS plate for VIA Freeze freezers

適用容器:Aseptic Technologies 2 mL vial 

總乘載量:15 × 2 mL Aseptic Technologies vial 

型號:ASY_30023

2 mL Nalgene vial SBS plate for VIA Freeze freezers

適用容器:Nalgene™ 2 mL vials 

總乘載量:48 × 2 mL Nalgene vial 

型號:ASY_30061

2 mL COOK vial SBS plate for VIA Freeze unit

適用容器:COOK 2 mL vials 

總乘載量:48 × 2 mL COOK vial 

型號:ASY_30065

VIA Freeze standard size vial SBS plate for 2 mL fill volumes

適用容器:Corning™ 2 mL vial (internally threaded), FluidX 2 mL vial 

總乘載量:48 × 2 mL standard size vial 

型號:ASY_30036

2 mL large vial SBS plate for VIA Freeze freezers

適用容器:NUNC™ 2 mL vials (internally threaded) 

總乘載量:48 × 2 mL large size vial 

型號:ASY_30062

2 mL extra large vial SBS plate for VIA Freeze freezers

適用容器:TBC

總乘載量:48 × 2 mL XL size vial 

型號:ASY_30086

2 mL large lid vial SBS plate for VIA Freeze freezers

適用容器:TBC

總乘載量:48 × 2 mL large lid vial 

型號:ASY_30087

2 mL extra extra large vial SBS plate for VIA Freeze freezers

適用容器:Greiner™ 2 mL vials 

總乘載量:48 × 2 mL XXL size vial 

型號:ASY_30054

2 mL West Pharma vial SBS plate for VIA Freeze freezers

適用容器:West Pharma 2 mL vials 

總乘載量:35 × 2 mL West Pharma™ vial 

型號:ASY_30088

5 mL West Pharma vial SBS plate for VIA Freeze freezers

適用容器:West Pharma 5 mL vials 

總乘載量:15 × 5 mL West Pharma™ vial 

型號:ASY_30097

VIA Freeze 6 mL Aseptic Technologies vial SBS plate

適用容器:Aseptic Technologies 6 mL vial 

總乘載量:12 × 6 mL Aseptic Technologies vial 

型號:ASY_30037

10 mL Aseptic Technologies vial SBS plate for VIA Freeze freezers

適用容器:Aseptic Technologies 10 mL vial 

總乘載量:12 × 10 mL Aseptic Technologies vial 

型號:ASY_30076

Straw

2 mm straw SBS plate for VIA Freeze freezers

適用容器:IMV 2 mm straws 

總乘載量:28 × 2 mm straw 

型號:ASY_30031

3 mm straw SBS plate for VIA Freeze freezers

適用容器:IMV 3 mm straws 

總乘載量:20 × 3 mm straw 

型號:ASY_30032

3.2 mm straw SBS plate for VIA Freeze freezers

適用容器:IMV 3.2 mm security straws 

總乘載量:20 × 3.2 mm straw 

型號:ASY_30078

Multiwell

Multiwell adapter for 96-well plates in VIA Freeze freezers

適用容器:Greiner™ 96 well plate, Corning™ 96 well plate 

總乘載量:1 × 96 well plate 

型號:ASY_30058

Bag

50 mL bag SBS plate for VIA Freeze freezers

適用容器:50 mL Origen bag, 50 mL CryoMACS™ bag 

總乘載量:1 bag

型號:ASY_30038

250 mL bag SBS adapter for VIA Freeze Duo

適用容器:250 mL Origen bag, 250 mL CryoMACS™ bag 

總乘載量:1 × 250 mL bag 

型號:ASY_30053

*(Duo機型限定)

250 mL bag SBS adapter for VIA Freeze Quad

適用容器:250 mL Origen bag, 250 mL CryoMACS™ bag 

總乘載量:2 × 250 mL bag 

型號:ASY_30070

*(Quad機型限定)

1000 mL bag SBS adapter for VIA Freeze Quad

適用容器:250, 500, or 1000 mL bag – Origen or CryoMACS™ 

總乘載量:1 bag 

型號:ASY_30066

*(Quad機型限定)

Cassette

VIA Freeze rack adapter for Biosafe bag cassettes

適用容器:Biosafe bag in cassette 

總乘載量:4 Biosafe bags in cassette 

型號:ASY_30080

VIA Freeze rack adapter for BioArchive bag cassettes

適用容器:BioArchive bag in cassette 

總乘載量:4 BioArchive™ bags in cassette 

型號:ASY_30081

VIA Freeze rack adapter for Aviamax bag cassettes

適用容器:Aviamax cassette 

總乘載量:4 Aviamax cassettes 

型號:ASY_30082

Video

Video

FAQs

FAQs

1.VIA Freeze™的運作原理?
VIA Freeze™是透過史特林冷凍機(Stirling Cryocooler)運行史特林循環(Stirling Cycle)冷卻樣品。這種冷凍機引擎因為具備高可靠度的特型常被用於遠程太空任務中,如NASA的RHESSI衛星。

2.容器形式是否有所限制?
我們目前有支援多種抗凍袋、抗凍管、Straw(請參考上方Plates and adapters for VIA Freeze)。此外,只要您的包裝形式能夠放入機器的冷凍室內,我們都可以幫您客製化生產SBS板與其連接使用。

3.VIA Freeze™可否過夜運作?
可以。您可以於前一日結束時開始運行,並於第二天早晨再取出樣品。

4.一個運行結束後需要間隔多久才能執行下一個循環?
VIA Freeze™會在運行結束後自動回溫、乾燥系統,通常會在60分鐘內完成。但可以根據需求加速。

5.系統可否同時冷卻袋裝及管裝樣本?
袋裝及管裝樣本可以同時進行冷凍,組合方式取決於所使用的系統型號。但是每個系統一次只能運行一組設定值。

6.VIA Freeze™溫控機制與液態氮降溫儀是否有所差異?
我們採用熱傳導方式進行樣品冷凍,這與基於液態氮的系統相比能提供更好的批次內與批次間一致性。

7.VIA Freeze™是否需要額外配置電腦?
VIA Freeze™能夠完全自主作業無需額外配置電腦,其內部配置有獨立運行的Linux作業系統,以確保安全性和可靠性。VIA Freeze™的操作可以藉由其配備的觸控螢幕。也可以透過Web頁面經由電腦、平板、手機進行遠端監控(可設定使用權限)。

References

References

1.GE Healthcare Life Sciences. Cryopreserve your samples with the VIA Freeze range of liquid nitrogen-free controlled rate freezers
本文章闡述VIA Freeze的作業機制、特性及優點。
前往文章

2.GE Healthcare Life Sciences. Controlled endpoint in cryopreservation.
樣本應該緩降溫到什麼溫度?本文章探討樣本緩降溫到不同溫度移轉到液態氮儲存桶長期儲存是否會產生不同效果…
前往文章

3.GE Healthcare Life Sciences. Delivering LN2-free large volume cryopreservation.
本文章探討袋裝樣本使用VIA Freeze執行緩降溫作業時,袋內每個位置是否能達到一致的降溫速率?抗凍袋在系統內呈水平或垂直擺放(可擺放更多樣本)是否會造成差異…
前往文章

4.GE Healthcare Life Sciences. Enabling LN2-free large-volume cryopreservation in vials.
本文章探討大容量的冷凍管裝樣本(50 ml冷凍管裝載30 ml樣本)在使用VIA Freeze進行降溫時,是否會因管徑厚度因素影響其降溫品質…
前往文章

5.GE Healthcare Life Sciences. Freezing of T cells using the VIA Freeze system.
本文章探討T Cell使用VIA Freeze、傳統液態氮降溫儀與降溫盒三者進行緩速降溫是否有所差異…
前往文章

6.GE Healthcare Life Sciences. Is rapid cooling step needed.
本文章探討在緩降溫過程中加入一驟降溫步驟(Rapid cooling step)與否對於樣品是否有所差異…
前往文章

7.BioLife Solutions. Process Optimization for CD3+ T-Cell Formulation and Cryopreservation.
本文章探討CD3+ T-Cell的細胞凍存,其中提及使用Asymptote VIA Freeze進行2mL冷凍小管緩降溫…
前往文章

8.Kilbride, Peter & Meneghel, Julie & Lamb, Stephen & Morris, George & Pouzet, Jerome & Jurgielewicz, Monika & Leonforte, Christopher & Gibson, Daniel & Madrigal, Alejandro. (2019). Recovery and Post-Thaw Assessment of Human Umbilical Cord Blood Cryopreserved as Quality Control Segments and Bulk Samples. Biology of Blood and Marrow Transplantation. 10.1016/j.bbmt.2019.09.004.
臍帶血抗凍袋保存時一般會將袋子的注入管再封口成多段以便日後檢驗使用。本文探討袋裝大樣本與注入管小樣本在解凍過程產生的差異。文中提及使用Asymptote VIA Freeze進行臍帶血線性緩降溫(2°C/min)…
前往文章

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實驗室最令人困擾的問題就在於空間不夠用,由於坪數有限,所以許多沒有規劃出獨立液態氮設備的儲存空間,又或者即便有儲存空間,空間也相當狹小、拘促…
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